Coding

Part:BBa_K1109002:Design

Designed by: Berkhan GENÇ   Group: iGEM13_Baskent_Meds   (2013-09-23)


lqsS-hdeD-lqsR coding region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 506
    Illegal PstI site found at 2664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 506
    Illegal PstI site found at 2664
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 506
    Illegal PstI site found at 2664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 506
    Illegal PstI site found at 2664
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2775


Design Notes

LqsR,hdeD and lqsS regions were amplified by PCR. Coding regions has unsuitable cutting areas so we amplified this region with one restriction site; speI.


Source

Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.)

References